DNA Extraction and Purification
Research Products International Corp ZymoPURE Plasmid Miniprep Kit, 50 Preps
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
Braintree Scientific Accuris Mouse Genotyping Kit, 1 hour, 400 reactions
Accuris™ 1 Hour Mouse/Mammalian Genotyping Kit DNA extraction and amplification in 1 hour Optimize proprietary lysis buffer Single tube - no organic solvents or clean up procedures Enhanced Accuris Hot Start Polymerase included The Accuris Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR Accuris Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers. **Sold for research purposes only and not for human use**
Biotium AccuGreen™ Broad Range dsDNA Quantitation Solution (500 assays)
The AccuGreen™ Broad Range dsDNA Quantitation Solution is designed for use with handheld fluorometers such as the Qubit fluorometer from Thermo Fisher. The AccuGreen™ quantitation solution is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL. This item is a standalone fluorescent DNA quantitation solution. The use of this product on the Qubit fluorometer requires two standards. The assay has a green fluorescence with an excitation/emission of 500/530 nm (with DNA). This product has sufficient reagent for 500 assays.
Magbio Genomics Inc HighPrep PCR (50mL)
HighPrep™ PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.
Zymo Research Corporation Urine Conditioning Buffer (8 ml)
Features- Effectively preserves DNA and RNA in urine atambient temperatures- Facilitates pelleting of both cellular and cell-freenucleic acids from large volume urine samples- Inhibits microbial growth during long-term (cold-free)storage of urine samples
Magbio Genomics Inc HighPrep Blood & Tissue DNA Kit (384 preps)
The HighPrep™ Blood & Tissue DNA kit is a magnetic beads based system to process high quality genomic DNA from 50-250 µL of fresh or frozen whole blood, buffy coat (containing anticoagulants such as Citrate, EDTA and Heparin), lysate of tissues, mouse tails, cultured cells, saliva, or buccal swabs.The HighPrep Blood & Tissue DNA kit is highly geared for automation where high quality genomic DNA is reproducible in a high throughput environment. The kit is adaptable to commercially available liquid handling workstations.
101 BIO Endotoxin-Free Plasmid Miniprep Kit
Eukaryotic cells are very sensitive to endotoxin, a common contaminant in the plasmid extraction, and the transfection efficiency of the cells will greatly reduce if the plasmid contains endotoxin. This kit can efficiently extract endotoxin-free plasmid in a fast and simple way. The endotoxin, genomic DNA, RNA, protein and other impurities can be largely removed. 3 ~30 μg transfection-grade plasmid DNA can be purified from 1 ~ 5 mL overnight culture of E. Coli in LB medium with this kit within 30 minutes. The extracted DNA can be directly used for Transfection PCR, restriction enzyme digestion, sequencing, etc. No need to precipitate, concentrate or desalt.
Biochain Institute Inc Genomic DNA - Plant Normal Tissue: Arabidopsis, 10 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc DNA Methylation Detection Kit, 1 kit/PK
BioChain's DNA methylation detection kit offers a fast, efficient and convenient alternative to traditional bisulfite conversions. Our kit provides users with a true endogenous human positive control which eliminates any ambiguity in validating results. The positive control has been tested in over 15 different human tissues. We have optimized our protocol to minimize DNA degradation, reagent preparation, and conversion time. Our protocol guarantees > 99 % conversion of cytosine to uracil and > 99 % CpG protection, as calculated by sequencing, and is sensitive over a range of 2 ug - 500 pg of DNA. In less than 3 hours, users can obtain high quality bisulfite modified DNA ready for analysis by PCR, sequencing, or microarrays.
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Skeletal Muscle, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Human Tumor Tissue: Thyroid Tumor, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Macherey-Nagel NucleoBond Xtra Maxi Plus, 50 preps
NucleoBond Xtra Maxi Plus (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A